Cell Line Authentication Using Isoenzyme Analysis

Strategies for accurate speciation and case studies for the detection of cell line cross-contamination using a commercial kit.

Confirmation of purity and identity of cell cultures is a necessary step in the production of biotherapeutics. Manipulation of multiple cell lines in the same facility introduces the possibility that cross-contamination may occur. Different cell lines may proliferate with varying growth rates such that a single cell from a rapidly growing line, introduced into a culture of slower growing cells, can overtake the original culture in the course of a few passages. In addition, cultures may be mislabeled during manipulation, again resulting in a …